Cardile V, Jiang X, Renis M, Bindoni M
Institute of Human Physiology, University of Catania, Italy.
Brain Res. 1996 Apr 9;715(1-2):98-103. doi: 10.1016/0006-8993(95)01564-7.
Ether lipid 1-O-octadecyl-2-O-methoxy-rac-glicero-3-phosphocholine (ET-18-OCH3) is an immunomodulator with antineoplastic activity. Its analog compounds PAF and CPAF share some of its biological effects. In our experiments, even very small amounts of ET-18-OCH3 released a remarkable quantity of nitric oxide (NO) from rat astrocytes cultured in vitro. The NO biosynthesis was inhibited by pretreatment with the antagonist BN 50730. The effect of ET-18-OCH3 was greater than that of the LPS inducer. PAF did not produce NO, even at high doses, while the nonmetabolizable CPAF only induced a significant release of NO from 12 micrograms/ml onwards. These results demonstrate that ET-18-OCH3 is functionally active also in astrocyte cultures. Stimulation of NO biosynthesis is of a great value on account of its the known effect as a neurotransmitter, potentiator of immune defences and possible modulator of cerebral circulation.
醚脂1-O-十八烷基-2-O-甲氧基-rac-甘油-3-磷酸胆碱（ET-18-OCH3）是一种具有抗肿瘤活性的免疫调节剂。其类似化合物血小板活化因子（PAF）和衣原体蛋白酶样活性因子（CPAF）具有一些与其相同的生物学效应。在我们的实验中，即使是极少量的ET-18-OCH3也能使体外培养的大鼠星形胶质细胞释放出大量的一氧化氮（NO）。一氧化氮的生物合成可被拮抗剂BN 50730预处理所抑制。ET-18-OCH3的作用比脂多糖诱导剂更强。即使高剂量的PAF也不会产生NO，而非代谢性的CPAF仅从12微克/毫升起才诱导出显著的NO释放。这些结果表明，ET-18-OCH3在星形胶质细胞培养物中也具有功能活性。由于一氧化氮作为神经递质、免疫防御增强剂以及可能的脑循环调节剂的已知作用，刺激其生物合成具有重要意义。
