Abnormal spermatozoa in the cauda epididymidis of adult and aged hamsters (Mesocricetus auratus): a study by electron microscopy.

The morphology of the spermatozoa in the cauda epididymidis of 6- and 24-month-old (adult and aged) hamster was studied by electron microscopy. Lesions found in the spermatozoa included alterations in the acrosomal matrix and nuclear membranes, abnormal or absent mitochondria, alterations in the axonemes, dense fibres and microtubules, and bent flagella. All these abnormalities are similar to those found in other species and few alterations were exclusive to the older animals. Ultrastructurally abnormal spermatozoa were observed in both groups but were present at a significantly greater frequency in aged hamsters (p < 0.005). the percentage of sperm with abnormal acrosomes, mitochondria and dense fibres and of bent (but not disrupted) spermatozoa was significantly greater in the older animals. These results show that the ultrastructural alterations in hamster sperm are similar to those found in other mammals. Moreover, the percentage of ultrastructurally abnormal spermatozoa in aged hamsters is greater than 6-month-old hamsters, this increase is not accompanied by any new kinds of alteration.