Toshimori K, Higashi R, Oura C
Department of Anatomy, Miyazaki Medical College, Japan.
Cell Tissue Res. 1987 Dec;250(3):673-80. doi: 10.1007/BF00218962.
The distribution of membrane filipin-sterol complexes (FSCs) was qualitatively surveyed on freeze-fracture replicas of spermatozoa from the male reproductive tract and ejaculates of golden hamster. In the head, the acrosomal plasma membrane showed the strongest filipin labeling on the principal segment, but it was absent in the "quilt-like pattern" areas. These latter were observed in both caput and corpus epididymal spermatozoa, but were absent in mature spermatozoa. The postacrosomal plasma membrane had few FSCs and both the outer and inner acrosomal membranes were always negative to filipin. The nuclear membrane of the principal segment was constantly filipin-positive. The nuclear membrane of the postacrosomal region had more FSCs than that of the principal segment, particularly in mature spermatozoa. Many linear, "rod-like FSCs" were observed on the postacrosomal nuclear membrane of mature spermatozoa, especially in the uterine spermatozoan samples. In the neck, the plasma membrane had only a few FSCs. The redundant nuclear membrane was slightly filipin-positive, while the membrane scroll of mature spermatozoa was heavily labeled. In the tail, the plasma membrane of both the middle and principal piece was moderately labeled.
对金黄仓鼠雄性生殖道精子和射精的冷冻断裂复制品上的膜制霉菌素-固醇复合物（FSCs）分布进行了定性研究。在头部，顶体细胞膜在主段显示最强的制霉菌素标记，但在“被子样模式”区域则没有。后者在附睾头和附睾体精子中均有观察到，但在成熟精子中不存在。顶体后细胞膜的FSCs很少，顶体外膜和内膜对制霉菌素始终呈阴性。主段的核膜始终呈制霉菌素阳性。顶体后区域的核膜比主段的核膜有更多的FSCs，特别是在成熟精子中。在成熟精子的顶体后核膜上观察到许多线性的“杆状FSCs”，尤其是在子宫内精子样本中。在颈部，细胞膜只有少量FSCs。多余的核膜呈轻度制霉菌素阳性，而成熟精子的膜卷则标记强烈。在尾部，中段和主段的细胞膜均有中度标记。
