丁基羟基茴香醚可阻断肿瘤坏死因子-α对人皮肤成纤维细胞胶原蛋白生成的抑制作用。

Butylated hydroxyanisole blocks the inhibitory effects of tumor necrosis factor-alpha on collagen production in human dermal fibroblasts.

作者信息

Taniguchi S, Furukawa M, Kono T, Hisa T, Ishii M, Hamada T

机构信息

Department of Dermatology, Osaka City University Medical School, Japan.

出版信息

J Dermatol Sci. 1996 Apr;12(1):44-9. doi: 10.1016/0923-1811(95)00463-7.

DOI:10.1016/0923-1811(95)00463-7

PMID:8740460

Abstract

Tumor necrosis factor-alpha (TNF-alpha) has been demonstrated to selectively decrease the production of type I and type III collagens in human dermal fibroblasts. The effects of the commonly used food antioxidants, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, propyl gallate, superoxide dismutase (SOD), and catalase on TNF-alpha-induced growth enhancement and collagen metabolism were evaluated in the present study. BHA at concentrations of both 5 x 10(-5) and 10(-4) M inhibited cell proliferation and DNA synthesis induced by 10 ng/ml TNF-alpha in human dermal fibroblasts, while other antioxidants had minimal effects. Further, BHA (5 x 10(-5) M and 10(-4) M) significantly blocked TNF-alpha-induced decreases in collagen synthesis. These results suggest that antioxidants such as BHA may be involved in the modulation of collagen synthesis by TNF-alpha in human dermal fibroblasts.

摘要

肿瘤坏死因子-α（TNF-α）已被证明可选择性降低人皮肤成纤维细胞中I型和III型胶原蛋白的产生。本研究评估了常用的食品抗氧化剂丁基羟基茴香醚（BHA）、丁基羟基甲苯（BHT）、α-生育酚、没食子酸丙酯、超氧化物歧化酶（SOD）和过氧化氢酶对TNF-α诱导的生长增强和胶原蛋白代谢的影响。浓度为5×10⁻⁵和10⁻⁴ M的BHA抑制了10 ng/ml TNF-α诱导的人皮肤成纤维细胞的细胞增殖和DNA合成，而其他抗氧化剂的影响极小。此外，BHA（5×10⁻⁵ M和10⁻⁴ M）显著阻断了TNF-α诱导的胶原蛋白合成减少。这些结果表明，诸如BHA之类的抗氧化剂可能参与了TNF-α对人皮肤成纤维细胞中胶原蛋白合成的调节。