Kawano M, Shinomiya M, Kanzaki T, Morisaki N, Shirai K, Saito Y, Yoshida S
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Clin Biochem. 1996 Jun;29(3):241-8. doi: 10.1016/0009-9120(96)00009-c.
To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages.
Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type lib hyperlipidemics were subjected to polyacrylamide gel disc electrophoresis.
A part of midband lipoprotein was observed between pre beta-and beta-band, in addition to the main beta-band. We named this midband lipoprotein "slow beta-migrating Lp (slow beta-Lp)." The larger LDL subfraction from midband-lipoprotein positive serum on Sepharose 2B column chromatography contained much slow beta-Lp, named slow beta-Lp-rich LDL. The smaller LDL subfraction contained a little slow beta-Lp, named slow beta-Lp-poor LDL. Slow beta-Lp-rich LDL had similar composition to the control LDL except for apolipoprotein E. The uptake of [3H]cholesteryl linoleate-labeled slow beta-Lp-rich LDL by J774 macrophages was higher than that of control LDL. The cholesterol ester content of J774 macrophages incubated with slow beta-Lp-rich LDL increased significantly compared with slow beta-Lp-poor LDL, beta-VLDL, and control LDL.
These results suggest that slow beta-Lp in type llb might generate foam cells from macrophages in atherosclerotic lesions.
阐明低密度脂蛋白（LDL）组分中的中带脂蛋白在与巨噬细胞相互作用时可能作为致动脉粥样硬化脂蛋白的可能性。
通过区带超速离心从Ⅱb型高脂血症患者中带脂蛋白阳性血清中分离出的低密度脂蛋白（LDL）进行聚丙烯酰胺凝胶圆盘电泳。
除主要的β带外，在前β带和β带之间观察到一部分中带脂蛋白。我们将这种中带脂蛋白命名为“慢β迁移脂蛋白（慢β-Lp）”。在琼脂糖2B柱色谱上，来自中带脂蛋白阳性血清的较大LDL亚组分含有大量慢β-Lp，称为富含慢β-Lp的LDL。较小的LDL亚组分含有少量慢β-Lp，称为慢β-Lp缺乏的LDL。除载脂蛋白E外，富含慢β-Lp的LDL与对照LDL具有相似的组成。J774巨噬细胞对[3H]胆固醇亚油酸酯标记的富含慢β-Lp的LDL的摄取高于对照LDL。与慢β-Lp缺乏的LDL、β-VLDL和对照LDL相比，与富含慢β-Lp的LDL孵育的J774巨噬细胞的胆固醇酯含量显著增加。
这些结果表明，Ⅱb型中的慢β-Lp可能在动脉粥样硬化病变中从巨噬细胞产生泡沫细胞。
