Bogitsh B J, Ribeiro-Rodrigues R, Carter C E
Department of Biology, Vanderbilt University, Nashville, Tennessee 37235, USA.
Parasitol Res. 1996;82(4):285-90. doi: 10.1007/s004360050115.
Trypanosoma cruzi epimastigotes were subjected to the lysosomotropic agents L-leucine methyl ester and ammonium chloride to determine their effects on the ultrastructure of the parasite. The lysosomotropic agents applied to epimastigotes caused a time-dependent alteration in the morphology of the cells marked by a 5-fold increase in the number of lysosomes. Continued exposure to ammonium chloride caused slight disruption of the reservosomes. The amino acid ester, however, while causing the parasite to swell after prolonged exposure (e.g., 24 h), had little effect on the reservosomes, the kinetoplast, or even the mitochondrion. A specific inhibitor of cysteine proteinases provided some protection for lysosomes from the effects of the amino acid ester. Although it is agreed that reservosomes are similar to endosomes, no lysosomal fusion with the reservosomes was observed. Acid phosphatase activity was observed only in lysosomes.
克氏锥虫前鞭毛体接受溶酶体亲和剂L-亮氨酸甲酯和氯化铵处理，以确定它们对寄生虫超微结构的影响。应用于前鞭毛体的溶酶体亲和剂导致细胞形态随时间发生改变，其特征是溶酶体数量增加了5倍。持续暴露于氯化铵会导致贮存体轻微破坏。然而，氨基酸酯虽然在长时间暴露（如24小时）后会使寄生虫肿胀，但对贮存体、动基体甚至线粒体几乎没有影响。半胱氨酸蛋白酶的特异性抑制剂为溶酶体提供了一定保护，使其免受氨基酸酯的影响。虽然人们一致认为贮存体类似于内体，但未观察到溶酶体与贮存体融合。酸性磷酸酶活性仅在溶酶体中观察到。
