Stitt A W, Fairweather I
School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland.
Parasitol Res. 1996;82(4):333-9. doi: 10.1007/s004360050122.
The effects of the active sulphoxide metabolite of the fasciolicide triclabendazole (Fasinex, Ciba-Geigy) on the vitelline cells of Fasciola hepatica were determined in vitro by transmission electron microscopy using both intact flukes and tissue-slice material. At a triclabendazole concentration of 15 micrograms/ml the vitelline cells of intact flukes showed ultrastructural changes only after prolonged incubation periods (12-24 h). The changes observed were a swelling of the granular endoplasmic reticulum (GER) cisternae with decreased ribosomal covering in the intermediate-type cells and condensation of chromatin and disappearance of the nucleolus in the nucleus of the stem cell. Similar changes were evident more quickly (by 6 h) in whole flukes treated at the higher concentration of 50 micrograms/ml. The shell globule clusters were loosely packed in the intermediate type-2 cells, and the number of intermediate type-1 cells declined with more prolonged incubation. Disruption of the nurse-cell cytoplasm was also observed from 12 h onwards. After only 6 h incubation of tissue-slice material at 50 micrograms/ml, intermediate type-1 cells were absent, shell globule clusters in mature cells were loosely packed and the nurse-cell cytoplasm was badly disrupted. By 12 h the vitelline cells were vacuolated and grossly abnormal. The results are discussed in relation to postulated actions of triclabendazole against the microtubule component of the cytoskeleton and against protein synthesis in the fluke.
通过透射电子显微镜，利用完整虫体和组织切片材料，在体外测定了杀片形吸虫药三氯苯达唑（肝蛭净，汽巴 - 嘉基公司）的活性亚砜代谢产物对肝片吸虫卵黄细胞的影响。在三氯苯达唑浓度为15微克/毫升时，完整虫体的卵黄细胞仅在长时间孵育（12 - 24小时）后才出现超微结构变化。观察到的变化包括中间型细胞中颗粒内质网（GER）池肿胀，核糖体覆盖减少，以及干细胞细胞核中染色质凝聚和核仁消失。在浓度为50微克/毫升的较高处理浓度下，完整虫体中类似变化出现得更快（6小时）。在中间2型细胞中，壳球簇排列松散，随着孵育时间延长，中间1型细胞数量减少。从12小时起还观察到营养细胞质的破坏。在50微克/毫升浓度下，组织切片材料仅孵育6小时后，中间1型细胞消失，成熟细胞中的壳球簇排列松散，营养细胞质严重破坏。到12小时时，卵黄细胞出现空泡化且严重异常。结合三氯苯达唑对吸虫细胞骨架微管成分和蛋白质合成的假定作用对结果进行了讨论。
