Iglesias R, Leiro J, Ubeira F M, Santamarina M T, Navarrete I, Sanmartín M L
Instituto Universitario de Investigación y Análisis Alimentarios, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Parasitol Res. 1996;82(4):378-81. doi: 10.1007/s004360050131.
We used ELISA and immunoblotting to investigate antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and five other nematodes: the ascaridoids Ascaris suum, Toxocara canis and Hysterothylacium aduncum, and the nonascaridoids Trichinella spiralis and Trichuris muris. Two sera were raised against each species (including A. simplex, but excluding A. suum), by infection or by immunization with somatic antigens. Serum against A. suum was raised by immunization only. The reactivities of each serum with A. simplex somatic antigens (SA), excretion-secretion antigens (ES), pseudocoelomic fluid antigens (PF) and cuticular antigens (CA) were investigated. The results of ELISA indicated high antigenic cross-reactivity between A. simplex and the remaining ascaridoid nematodes, confirming that there is extensive antigenic similarity within this group of nematode parasites. Immunoblotting again confirmed the high degree of cross-reactivity between the SA of A. simplex and SAs of the other ascaridoids, although several A. simplex SA components in the 11-18 kDA range were only recognized by sera from mice infected with A. simplex. In addition, two A. simplex PF components of 22 and 27 kDA, were recognized only by sera from mice infected with, or immunized with the SA of, A. simplex. Finally, the anti-phosphorylcholine monoclonal antibody BH8 recognized only a small number of A. simplex antigens, indicating that phosphorylcholine epitopes are not significant contributors to the observed cross-reactivity with the other nematodes.
我们使用酶联免疫吸附测定法（ELISA）和免疫印迹法，研究了简单异尖线虫三期幼虫与其他五种线虫在小鼠体内的抗原交叉反应性：蛔虫类的猪蛔虫、犬弓首蛔虫和钩状异唇线虫，以及非蛔虫类的旋毛虫和鼠鞭虫。通过感染或用体细胞抗原免疫，针对每个物种（包括简单异尖线虫，但不包括猪蛔虫）制备了两份血清。仅通过免疫制备了针对猪蛔虫的血清。研究了每种血清与简单异尖线虫体细胞抗原（SA）、排泄-分泌抗原（ES）、假体腔液抗原（PF）和表皮抗原（CA）的反应性。ELISA结果表明，简单异尖线虫与其余蛔虫类线虫之间存在高度的抗原交叉反应性，证实了这组线虫寄生虫内部存在广泛的抗原相似性。免疫印迹再次证实了简单异尖线虫的SA与其他蛔虫类线虫的SA之间存在高度的交叉反应性，尽管11-18 kDa范围内的几种简单异尖线虫SA成分仅被感染简单异尖线虫的小鼠血清识别。此外，22 kDa和27 kDa的两种简单异尖线虫PF成分仅被感染简单异尖线虫或用其SA免疫的小鼠血清识别。最后，抗磷酸胆碱单克隆抗体BH8仅识别少数简单异尖线虫抗原，表明磷酸胆碱表位对观察到的与其他线虫的交叉反应性贡献不大。
