Iglesias R, Leiro J, Ubeira F M, Santamarina M T, Sanmartín M L
Instituto de Investigación y Análisis Alimentario, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
J Helminthol. 1995 Dec;69(4):319-24. doi: 10.1017/s0022149x00014899.
To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion-secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS-PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion-secretion and surface antigen preparations from third-stage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion-secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis.
为研究简单异尖线虫发育阶段之间的抗原差异，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法，以含有针对两个幼虫阶段抗体的小鼠免疫血清对第三期和第四期简单异尖线虫幼虫的虫体、排泄分泌产物及去污剂增溶表面抗原制剂，以及成虫的虫体抗原制剂进行了表征。在SDS-PAGE和免疫印迹中，第三期幼虫的排泄分泌产物和表面抗原制剂与第四期幼虫的表现差异很大；这表明某些排泄分泌产物和表面抗原具有高度的阶段特异性。相比之下，发现第三期和第四期幼虫的虫体成分具有非常相似的条带模式，这表明在寄生虫在小鼠体内发育过程中这些成分具有保守性。成虫虫体成分的SDS-PAGE和免疫印迹结果似乎支持这一假设。
