4-五氟乙基伞形酮基-β-D-葡萄糖苷作为酸性β-D-葡萄糖苷酶的新型荧光底物。

4-Pentafluoroethylumbelliferyl-beta-D-glucoside as a new fluorogenic substrate for acid beta-D-glucosidase.

作者信息

Tsvetkova I V, Karpova E A, Dudukina T V, Voznyi Y V

机构信息

Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.

出版信息

Clin Chim Acta. 1996 Apr 30;248(2):125-33. doi: 10.1016/0009-8981(95)06246-7.

DOI:10.1016/0009-8981(95)06246-7

PMID:8740577

Abstract

4-Pentafluoroethylumbelliferyl-beta-D-glucoside is proposed as an efficient substrate for human leukocyte acid beta-glucosidase. Its synthesis is described. This substrate was compared directly with 4-trifluoromethylumbelliferyl-beta-D-glucoside synthesized by us earlier and with 4-methylumbelliferyl-beta-D-glucoside which is commonly used for acid beta-glucosidase activity assay. The specific activity of acid beta-glucosidase with 4-pentafluoroethylumbelliferyl-beta-D-glucoside was 3- and 8-fold higher than it was with the substrates mentioned above. The kinetic parameters KM and VMAX for human leukocyte acid beta-glucosidase with the three substrates was determined. One possible application of the newly synthesized substrate is its use in the diagnosis of acid beta-glucosidase hereditary deficiency (Gaucher's disease).

摘要

4-五氟乙基伞形酮基-β-D-葡萄糖苷被提议作为人白细胞酸性β-葡萄糖苷酶的一种有效底物。文中描述了其合成过程。将该底物与我们之前合成的4-三氟甲基伞形酮基-β-D-葡萄糖苷以及常用于酸性β-葡萄糖苷酶活性测定的4-甲基伞形酮基-β-D-葡萄糖苷直接进行了比较。酸性β-葡萄糖苷酶对4-五氟乙基伞形酮基-β-D-葡萄糖苷的比活性分别比上述底物高3倍和8倍。测定了人白细胞酸性β-葡萄糖苷酶对这三种底物的动力学参数KM和VMAX。新合成底物的一种可能应用是用于诊断酸性β-葡萄糖苷酶遗传性缺乏症（戈谢病）。