Barroso C P, Edvinsson L, Zhang W, Cunha e Sá M, Springall D R, Polak J M, Gulbenkian S
Department of Cell Biology, Gulbenkian Institute of Science, Oeiras, Portugal.
J Auton Nerv Syst. 1996 Apr 20;58(1-2):108-14. doi: 10.1016/0165-1838(95)00134-4.
The presence and distribution of nitric oxide synthase (NOS)-immunoreactive nerve fibers associated with the guinea pig major cerebral arteries was studied by means of immunohistochemical, histochemical and ultrastructural techniques. Anterior arteries of the circle of Willis received a rich supply of perivascular nerve fibers containing NOS immunoreactivity while posteriorly localized arteries presented a moderate to sparse innervation. A double immunofluorescence staining technique revealed that NOS was localized in nerve fibers distinct from those displaying substance P or tyrosine hydroxylase. Combined immunofluorescence and histochemical staining of the same preparation indicated that NOS immunoreactivity was localized in putative cholinergic nerve fibers (identified by their acetylcholinesterase content) and that NADPH-diaphorase activity (a marker for NOS-containing neurons) was found in nerves which also possessed VIP immunoreactivity. The ultrastructural study revealed that NOS immunoreactivity was present in numerous nerve varicosities at the adventitial-medial border. These results suggest that NO and VIP co-exist in putative parasympathetic nerve fibers supplying the guinea pig cerebral arteries and may be release together in response to nervous stimulation.
采用免疫组织化学、组织化学和超微结构技术，研究了豚鼠大脑主要动脉中一氧化氮合酶（NOS）免疫反应性神经纤维的存在和分布情况。 Willis环的前动脉接受了丰富的含NOS免疫反应性的血管周围神经纤维供应，而后部动脉的神经支配则为中度至稀疏。双重免疫荧光染色技术显示，NOS定位于与显示P物质或酪氨酸羟化酶的神经纤维不同的神经纤维中。对同一标本进行免疫荧光和组织化学联合染色表明，NOS免疫反应性定位于假定的胆碱能神经纤维（根据其乙酰胆碱酯酶含量确定），并且在也具有VIP免疫反应性的神经中发现了NADPH-黄递酶活性（含NOS神经元的标志物）。超微结构研究显示，在中膜-外膜边界的许多神经膨体中存在NOS免疫反应性。这些结果表明，NO和VIP共存于供应豚鼠脑动脉的假定副交感神经纤维中，并且可能在神经刺激下一起释放。
