间接免疫荧光法和免疫印迹法检测地方性落叶型天疱疮（fogo selvagem）细胞间抗体的敏感性。
Sensitivity of indirect immunofluorescence and immunoblotting for the detection of intercellular antibodies in endemic pemphigus foliaceus (fogo selvagem).
作者信息
Rowilson-Cunha P, Bystryn J C
机构信息
Ronald O. Perelman Department of Dermatology, New York University Medical Center, New York 10015, USA.
出版信息
Int J Dermatol. 1999 Jan;38(1):41-5. doi: 10.1046/j.1365-4362.1999.00568.x.
BACKGROUND
Two assays are available to detect anti-skin antibodies in patients with fogo selvagem (FS): indirect immunofluorescence (IIF) and immunoblotting (IB). This study was conducted to compare the sensitivity of these assays in detecting FS antibodies.
DESIGN
Eighty-nine serum samples of 48 patients with FS and control serum from 15 normal individuals were tested concurrently for the presence of FS antibodies by IIF and IB. IIF studies were conducted using four different substrates: human skin, monkey and guinea pig esophagus, and bovine tongue.
RESULTS
FS antibodies were detected much more commonly by IIF than by IB, i.e. in 71% vs. 28% of serum samples respectively. By IIF, the antibodies reacted most strongly against human skin.
CONCLUSIONS
IIF is a more sensitive assay than IB for detecting antibodies associated with FS. The sensitivity of the test is maximized by using human skin as a substrate.
背景
有两种检测巴西美洲利什曼病（FS）患者抗皮肤抗体的检测方法：间接免疫荧光法（IIF）和免疫印迹法（IB）。本研究旨在比较这些检测方法在检测FS抗体方面的敏感性。
设计
同时采用IIF和IB检测48例FS患者的89份血清样本以及15名正常个体的对照血清中FS抗体的存在情况。IIF研究使用了四种不同的底物：人皮肤、猴和豚鼠食管以及牛舌。
结果
通过IIF检测到FS抗体的情况比IB更为常见，即分别在71%和28%的血清样本中检测到。通过IIF，抗体与人皮肤的反应最为强烈。
结论
在检测与FS相关的抗体方面，IIF比IB更敏感。使用人皮肤作为底物可使检测的敏感性最大化。
Zotero 插件