Creemers P C, Du Toit E D, Kriel J
Provincial Laboratory for Tissue Immunology, Observatory, Cape Town, South Africa.
Gene Geogr. 1995 Dec;9(3):185-9.
The genetic polymorphism of vitamin D binding protein (DBP) and of properdin Factor B (BF) was determined in unrelated Namibian San and Khoi, and in South African Blacks, Caucasoids and Cape Coloureds. Alleles have been confirmed by segregation patterns in family studies. The DBP phenotypes were identified by isoelectric focusing on ultrathin polyacrylamide gels and the BF phenotypes were identified by electrophoresis on 1% agarose gels; both methods were followed by immunofixation. The DBP and BF allele frequencies for all population groups were found to be in accordance with Hardy-Weinberg equilibrium. DBP1S and BFS allele frequencies in the San, Khoi and Blacks were similar; their frequency was far lower than in Caucasoids. The frequencies of the DBP1F and BFF were also similar in the San, Khoi and Blacks; however, the allele frequency was much higher in these groups than in Caucasoids. These differences were statistically significant (P < 0.001).
在纳米比亚的桑族和科伊族以及南非黑人、白种人和开普混血儿中，对维生素D结合蛋白（DBP）和备解素因子B（BF）的基因多态性进行了测定。通过家系研究中的分离模式对这些等位基因进行了确认。DBP表型通过在超薄聚丙烯酰胺凝胶上的等电聚焦进行鉴定，BF表型通过在1%琼脂糖凝胶上的电泳进行鉴定；两种方法之后都进行免疫固定。发现所有人群组的DBP和BF等位基因频率均符合哈迪-温伯格平衡。桑族、科伊族和黑人中DBP1S和BFS等位基因频率相似；其频率远低于白种人。桑族、科伊族和黑人中DBP1F和BFF的频率也相似；然而，这些组中的等位基因频率远高于白种人。这些差异具有统计学意义（P < 0.001）。
