A new BF F variant by polyacrylamide gel isoelectric focusing.

Polyacrylamide gel isoelectric focusing followed by electroblotting with enzyme immunoassay was done for the investigation of allotypes of properdin factor B (BF) in serum from 326 Japanese subjects. A new BF F variant tentatively designated BFFb1 (b = basic) was detected, the isoelectric point of each band of homozygous BF Fb1 being higher than of BF FF. Family data were in accordance with transmission by mendelian inheritance. The allele frequencies calculated from 326 Japanese subjects were 0.7945, 0.1825, 0.0215, and 0.0015 for BFS, BFF, BFFb1, and BFF075, respectively, with that of variant BFFb1 being a polymorphic frequency. The distribution of phenotypes fitted the Hardy-Weinberg equilibrium.