[培养的鸡视网膜色素上皮细胞中前列腺素D2的产生与代谢]

[Production and metabolism of prostaglandin D2 by cultured chick retinal pigment epithelial cells].

作者信息

Shiroma T, Yamakawa R, Yamashiro A, Nagataki S

机构信息

Department of Ophthalmology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1996 Jul;100(7):501-6.

PMID:8741332

Abstract

The production and metabolism of prostaglandin (PG) D2 in cultured chick retinal pigment epithelial (RPE) cells were studied. The production of PGD2 was evaluated using thin layer chromatography (TLC) after labeling the RPE cells with 1-14Carachidonic acid (AA) and radioimmunoassay (RIA). The metabolism was evaluated by measuring the activity of 15-hydroxyprostaglandin dehydrogenase. The conversion of AA to PGD2 was detected by TLC, and this conversion was inhibited with indomethacin (10(-5)M). In a steady state, 198 +/- 50 pg/6 x 10(5) cells/2 hours of PGD2 was detected by RIA. The amount of PGD2 was greater than that of both PGE2 and PGF2 alpha. The nicotinamide adenine dinucleotide phosphate (NADP)-linked 15-hydroxyprostaglandin dehydrogenase activity was detected in the 10,000 g supernatant of the RPE cells. These results demonstrated that cultured chick RPE cells produce and metabolize PGD2.

摘要

研究了培养的鸡视网膜色素上皮（RPE）细胞中前列腺素（PG）D2的产生和代谢。在用1-14C花生四烯酸（AA）标记RPE细胞后，使用薄层色谱法（TLC）和放射免疫测定法（RIA）评估PGD2的产生。通过测量15-羟基前列腺素脱氢酶的活性来评估代谢。通过TLC检测到AA向PGD2的转化，并且这种转化被吲哚美辛（10（-5）M）抑制。在稳态下，通过RIA检测到198±50 pg/6×10（5）个细胞/2小时的PGD2。PGD2的量大于PGE2和PGF2α的量。在RPE细胞的10,000g上清液中检测到烟酰胺腺嘌呤二核苷酸磷酸（NADP）连接的15-羟基前列腺素脱氢酶活性。这些结果表明，培养的鸡RPE细胞产生并代谢PGD2。