Ishizaka A, Suzuki Y, Kanazawa M, Fujita H, Kawashiro T, Yokoyama T
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1993 Jan;31(1):51-8.
We investigated the effects of various doses (0.2 to 2000 micrograms/kg) of endotoxin on physiological and hematological parameters in our guinea pig model. Bronchoalveolar lavage (BAL) and lung tissue sampling were performed 6 hours after endotoxin injection. Lung damage was assessed by measuring wet to dry lung weight ratio (W/D), lung tissue to plasma accumulation of 125I-albumin (AL: albumin leakage) and BAL fluid to plasma accumulation of 125I-albumin (BALL: bronchoalveolar lavage leakage). Changes in peripheral cell counts were estimated at times 0, 1, 2, 4 and 6 hours after endotoxin injection. Pulmonary cell accumulation was determined by counting the number of cells in BAL fluid and tissue samples fixed for light microscopic examination. Increased W/D and AL were observed only in the high dose endotoxin treatment groups (200 and 2000 micrograms/kg), while BALL increased even in the low dose endotoxin treatment groups (2 micrograms/kg). Decreases in peripheral neutrophil counts at one hour were observed in all endotoxin treated groups. Increase in BAL fluid neutrophils was seen in the 20, 200, and 2000 micrograms/kg groups, while neutrophil accumulation assessed by light microscope was observed in all endotoxin treated groups. We conclude that different amounts of endotoxin are required to affect different parameters of lung injury.
我们在豚鼠模型中研究了不同剂量（0.2至2000微克/千克）内毒素对生理和血液学参数的影响。在内毒素注射6小时后进行支气管肺泡灌洗（BAL）和肺组织采样。通过测量肺湿重与干重之比（W/D）、肺组织中125I-白蛋白的血浆蓄积量（AL：白蛋白渗漏）以及支气管肺泡灌洗液中125I-白蛋白的血浆蓄积量（BALL：支气管肺泡灌洗渗漏）来评估肺损伤。在内毒素注射后0、1、2、4和6小时估计外周血细胞计数的变化。通过对固定用于光学显微镜检查的BAL液和组织样本中的细胞进行计数来确定肺细胞蓄积情况。仅在高剂量内毒素治疗组（200和2000微克/千克）中观察到W/D和AL增加，而即使在低剂量内毒素治疗组（2微克/千克）中BALL也增加。在所有内毒素治疗组中均观察到1小时外周中性粒细胞计数减少。在20、200和2000微克/千克组中可见BAL液中性粒细胞增加，而在所有内毒素治疗组中通过光学显微镜评估均观察到中性粒细胞蓄积。我们得出结论，影响肺损伤的不同参数需要不同量的内毒素。
