Menezes J R, Smith C M, Nelson K C, Luskin M B
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Mol Cell Neurosci. 1995 Dec;6(6):496-508. doi: 10.1006/mcne.1995.0002.
In the mammalian forebrain most neurons originate from proliferating cells in the ventricular zone lining the lateral ventricles. These neurons become postmitotic before they undergo migration to their final destinations. In this study we examined the proliferative and migratory properties of cells destined for the olfactory bulb that arise postnatally from progenitor cells situated at the anterior extent of the subventricular zone (SVZa). The SVZa-derived cells migrate along a stereotypical pathway to the olfactory bulb where they become interneurons. Using lineage tracers and the cell proliferation marker BrdU, we have demonstrated that SVZa-derived cells in the rat retain the capacity for division after migrating away from their initial site of generation. These cells also express a neuron-specific tubulin, recognized by the antibody TuJ1. These results suggest that, unlike other immature neurons, these SVZa-derived cells have made a commitment to become neurons before becoming postmitotic.
在哺乳动物的前脑，大多数神经元起源于侧脑室壁室管膜区的增殖细胞。这些神经元在经历迁移至其最终目的地之前就进入了有丝分裂后期。在本研究中，我们检测了出生后源自位于脑室下区（SVZa）前部的祖细胞、 destined for the olfactory bulb的细胞的增殖和迁移特性。源自SVZa的细胞沿着一条刻板的路径迁移至嗅球，在那里它们成为中间神经元。使用谱系追踪剂和细胞增殖标记物BrdU，我们已经证明，大鼠中源自SVZa的细胞在从其最初产生部位迁移后仍保留分裂能力。这些细胞还表达一种神经元特异性微管蛋白，可被抗体TuJ1识别。这些结果表明，与其他未成熟神经元不同，这些源自SVZa的细胞在进入有丝分裂后期之前就已决定成为神经元。
