O'Flaherty J T, Kreutzer D L, Ward P A
J Immunol. 1977 Jul;119(1):232-9.
An assay is described for measuring neutrophil aggregation and cell size with a Coulter Counter equipped with a mean cell volume computer and volume channalyzer. With this assay, it was found that several chemotactically active agents rapidly induce rabbit peritoneal polymorphonuclear neutrophils in suspension to aggregate reversibly. The aggregating activities of these chemotactic agents paralleled their in vitro chemotactic- and in vivo neutropenia-inducing potencies. Neutrophil aggregation may underlie the neutropenia which follows the infusion of these agents into rabbits. Chemotactic agents may also induce polymorphonuclear neutrophils to increase in volume; this effect appears to be independent of aggregation. The volume change develops more slowly and does not appear reversible under the conditions employed. The volume increase may be an expression of pseudopodia formation and membrane ruffling known to develop in neutrophils exposed to chemotactic agents.
本文描述了一种使用配备平均细胞体积计算机和体积分析仪的库尔特计数器测量中性粒细胞聚集和细胞大小的检测方法。通过该检测方法发现，几种趋化活性物质能迅速诱导悬浮状态下的兔腹膜多形核中性粒细胞发生可逆性聚集。这些趋化剂的聚集活性与其体外趋化和体内诱导中性粒细胞减少的效力平行。中性粒细胞聚集可能是这些物质注入兔体内后导致中性粒细胞减少的原因。趋化剂还可能诱导多形核中性粒细胞体积增大；这种效应似乎与聚集无关。体积变化发展得更缓慢，在所采用的条件下似乎不可逆。体积增加可能是中性粒细胞在接触趋化剂时形成伪足和膜皱褶的一种表现。
