Hashimoto Y, Hurd E R
Clin Exp Immunol. 1981 Jun;44(3):538-47.
Several types of IgG-dependent phagocytic stimuli independent of complement were investigated for their property to induce human polymorphonuclear neutrophil leucocyte (PMN) aggregation and adherence to human endothelial cells (EC) in culture. A Coulter counter method was employed for the detection of cell aggregation. Aggregated IgG, ovalbumin-anti-ovalbumin (OV anti-OV) immune complexes (both insoluble and soluble) and opsonized latex particles induced a significant degree of PMN aggregation which was detectable as early as 2 min after exposure of PMN to these stimuli. This aggregation was dependent on divalent cations (Ca++, Mg++). The same phagocytic stimuli furthermore significantly increased adherence of PMN to cultured human EC and serum-coated plastic. Controls consisting of native IgG, and OV anti-OV complexes prepared from Fab')2 antibody failed to induce either aggregation or increased adherence of PMN. These data suggest that exposure of PMN to IgG-dependent phagocytic stimuli induces increased adhesiveness of PMN and that interaction between the Fc-receptor of PMN and the Fc-portion of phagocytic stimuli is essential for this effect.
研究了几种不依赖补体的IgG依赖性吞噬刺激物在培养中诱导人多形核中性粒细胞（PMN）聚集以及与人内皮细胞（EC）黏附的特性。采用库尔特计数器法检测细胞聚集。聚集的IgG、卵清蛋白-抗卵清蛋白（OV抗OV）免疫复合物（包括不溶性和可溶性）以及调理的乳胶颗粒可诱导显著程度的PMN聚集，早在PMN暴露于这些刺激物后2分钟即可检测到。这种聚集依赖于二价阳离子（Ca++、Mg++）。同样的吞噬刺激物还显著增加了PMN对培养的人EC和血清包被塑料的黏附。由天然IgG以及由Fab'2抗体制备的OV抗OV复合物组成的对照未能诱导PMN聚集或增加其黏附。这些数据表明，PMN暴露于IgG依赖性吞噬刺激物会诱导PMN黏附性增加，并且PMN的Fc受体与吞噬刺激物的Fc部分之间的相互作用对于这种效应至关重要。
