从具有甲硫氨酸反密码子CAT的tDNA推导的枯草芽孢杆菌次要异亮氨酸tRNA的特性分析
Characterization of a B. subtilis minor isoleucine tRNA deduced from tDNA having a methionine anticodon CAT.
作者信息
Matsugi J, Murao K, Ishikura H
机构信息
Laboratory of Chemistry, Jichi Medical School, Tochigi.
出版信息
J Biochem. 1996 Apr;119(4):811-6. doi: 10.1093/oxfordjournals.jbchem.a021312.
Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine. We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC, UV, and FAB mass spectroscopy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-beta-d-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.
枯草芽孢杆菌属于革兰氏阳性真细菌，据预测，它有一种次要的异亮氨酸转运核糖核酸（tRNA），由具有CAT反密码子的基因转录而来，该反密码子对应于甲硫氨酸。我们分离出了这种tRNA，并确定了其包括修饰核苷酸在内的序列。使用薄层层析（TLC）、紫外光（UV）和快原子轰击（FAB）质谱进行的修饰核苷酸分析表明，反密码子的第一个字母被修饰为赖氨酸idine [4-氨基-2-(N6-赖氨酸基)-1-β-D-呋喃核糖基嘧啶]。结果，这种tRNA与从DNA序列预测的次要tRNA一致，并被认为可解码异亮氨酸密码子AUA。
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