Weber F, Dietrich A, Weil J H, Maréchal-Drouard L
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Nucleic Acids Res. 1990 Sep 11;18(17):5027-30. doi: 10.1093/nar/18.17.5027.
The nucleotide sequence of a potato mitochondrial tRNA(Ile) has been determined. This tRNA has a lysidine-like minor nucleotide in the first position of the anticodon (position 34). The corresponding mitochondrial gene has been cloned and sequenced. This gene has a CAT anticodon which corresponds to methionine. The C residue in the anticodon must therefore be post-transcriptionally modified. The mature tRNA has isoleucine-accepting activity but no methionine-accepting activity. This is the first report showing that in plant mitochondria a post-transcriptional modification could change the amino-acid specificity of a tRNA, as compared to that of the corresponding gene.
已确定马铃薯线粒体tRNA（Ile）的核苷酸序列。该tRNA在反密码子的第一位（第34位）含有一个类似赖氨酸idine的次要核苷酸。相应的线粒体基因已被克隆并测序。该基因具有与甲硫氨酸对应的CAT反密码子。因此，反密码子中的C残基必须进行转录后修饰。成熟的tRNA具有异亮氨酸接受活性，但没有甲硫氨酸接受活性。这是第一份报告表明，与相应基因相比，在植物线粒体中，转录后修饰可以改变tRNA的氨基酸特异性。
