Adams M D, Rudner D Z, Rio D C
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Curr Opin Cell Biol. 1996 Jun;8(3):331-9. doi: 10.1016/s0955-0674(96)80006-8.
During the past year, significant advances have been made in the field of pre-mRNA splicing. It is now clear that members of the serine-arginine-rich protein family are key players in exon definition and function at multiple steps in the spliceosome cycle. Novel findings have been made concerning the role of exon sequences, which function as both constitutive and regulated enhancers of splicing, in trans-splicing and as targets for tissue-specific control of splicing patterns. By combining biochemical approaches in human and yeast extracts with genetic analysis, much has been learned about the RNA-RNA and RNA-protein interactions that are necessary to assemble the various complexes that are found along the pathway to the catalytically active spliceosome.
在过去的一年里，前体mRNA剪接领域取得了重大进展。现在已经清楚的是，富含丝氨酸-精氨酸的蛋白质家族成员是外显子定义和剪接体循环多个步骤中功能的关键参与者。关于外显子序列的作用有了新的发现，外显子序列在反式剪接中既作为组成型又作为受调控的剪接增强子发挥作用，并且是剪接模式组织特异性控制的靶点。通过将人类和酵母提取物中的生化方法与遗传分析相结合，我们对沿着通往具有催化活性的剪接体的途径中发现的各种复合物组装所必需的RNA-RNA和RNA-蛋白质相互作用有了很多了解。
