Rohrer G A, Alexander L J, Hu Z, Smith T P, Keele J W, Beattie C W
U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (MARC), Clay Center, Nebraska 68933-0166, USA.
Genome Res. 1996 May;6(5):371-91. doi: 10.1101/gr.6.5.371.
We report the highest density genetic linkage map for a livestock species produced to date. Three published maps for Sus scrofa were merged by genotyping virtually every publicly available microsatellite across a single reference population to yield 1042 linked loci, 536 of which are novel assignments, spanning 2286.2 cM (average interval 2.23 cM) in 19 linkage groups (18 autosomal and X chromosomes, n = 19). Linkage groups were constructed de novo and mapped by locus content to avoid propagation of errors in older genotypes. The physical and genetic maps were integrated with 123 informative loci assigned previously by fluorescence in situ hybridization (FISH). Fourteen linkage groups span the entire length of each chromosome. Coverage of chromosomes 11, 12, 15, and 18 will be evaluated as more markers are physically assigned. Marker-deficient regions were identified only on 11q1.7-qter and 14 cen-q1.2. Recombination rates (cM/Mbp) varied between and within chromosomes. Short chromosomal arms recombined at higher rates than long arms, and recombination was more frequent in telomeric regions than in pericentric regions. The high-resolution comprehensive map has the marker density needed to identify quantitative trait loci (QTL), implement marker-assisted selection or introgression and YAC contig construction or chromosomal microdissection.
我们报告了迄今为止构建的家畜物种的最高密度遗传连锁图谱。通过对单个参考群体中几乎所有公开可用的微卫星进行基因分型，合并了已发表的3个猪（Sus scrofa）图谱，得到1042个连锁基因座，其中536个是新定位的，分布在19个连锁群（18个常染色体和X染色体，n = 19）中，跨度为2286.2厘摩（平均间距2.23厘摩）。连锁群是从头构建的，并根据基因座内容进行定位，以避免旧基因型中的错误传播。物理图谱和遗传图谱通过先前通过荧光原位杂交（FISH）定位的123个信息位点进行整合。14个连锁群覆盖了每条染色体的全长。随着更多标记物进行物理定位，将对11、12、15和18号染色体的覆盖情况进行评估。仅在11q1.7 - qter和14 cen - q1.2上鉴定出标记物缺乏区域。染色体之间和染色体内的重组率（厘摩/兆碱基对）有所不同。短染色体臂的重组率高于长染色体臂，端粒区域的重组比着丝粒周围区域更频繁。该高分辨率综合图谱具有鉴定数量性状基因座（QTL）、实施标记辅助选择或基因渗入以及酵母人工染色体（YAC）重叠群构建或染色体显微切割所需的标记密度。
