Roldán-Villalobos R, Artacho-Pérula E, Ruiz-Moruno F J
Department of Morphological Sciences, School of Medicine, University of Córdoba, Spain.
Anal Quant Cytol Histol. 1996 Apr;18(2):158-66.
Quantitative evaluation of nuclear size in breast cancer is performed with the aim of investigating whether the data obtained are related to subjective grading and prognosis.
Unbiased stereologic estimates of the volume-weighted mean nuclear volume (nuclear vv) in paraffin-embedded tissue from 44 specimens of infiltrating ductal breast carcinoma were obtained by the point-sampled intercepts method.
Histologically graded cases showed an overlap in nuclear vv estimates, but significant differences were demonstrated in which averaged values increased from grade 1 to 3 breast carcinomas. Nuclear vv estimates in grade 3 breast carcinoma (mean, 495.4 microns 3; SD, 101.7 microns 3) were significantly larger than those in grades 1 (mean, 327.8 microns 3; SD, 90.9 microns 3) and 2 (mean, 409.9 microns 3; SD, 72.6 microns 3) (P < .01). Minor statistically significant differences (P < .05) were found between grades 1 and 2. Estimates of nuclear vv based on systematic sampling within the specimen showed high efficiency, with > 80% of the total observed variance contributing to biologic differences between individual specimens. Univariate prognostic study showed that clinical stage, and not histologic grade, was of prognostic value in the cases studied. Similarly, nuclear vv estimates were associated with disease recurrence and survival, with a cutoff point of 425 microns 3.
Based on the cases investigated, shape-independent nuclear vv estimates may be an adjuvant tool in the grading and prognostic evaluation of infiltrating ductal breast carcinoma.
对乳腺癌细胞核大小进行定量评估，旨在研究所得数据是否与主观分级及预后相关。
采用点抽样截距法，对44例浸润性导管癌石蜡包埋组织中的体积加权平均核体积（核vv）进行无偏体视学估计。
组织学分级病例的核vv估计值存在重叠，但显示出显著差异，即平均核vv值从1级到3级乳腺癌逐渐增加。3级乳腺癌的核vv估计值（均值为495.4立方微米；标准差为101.7立方微米）显著大于1级（均值为327.8立方微米；标准差为90.9立方微米）和2级（均值为409.9立方微米；标准差为72.6立方微米）（P <.01）。1级和2级之间存在微小的统计学显著差异（P <.05）。基于标本内系统抽样的核vv估计显示出高效性，总观察方差的80%以上可归因于个体标本之间的生物学差异。单因素预后研究表明，在所研究的病例中，临床分期而非组织学分级具有预后价值。同样，核vv估计值与疾病复发和生存相关，截断点为425立方微米。
基于所研究的病例，与形状无关的核vv估计可能是浸润性导管癌分级和预后评估的辅助工具。
