Lacki J K, Klama K, Porawska W, Mackiewicz S H, Müller W, Wiktorowicz K
Department of Clinical Immunology and Allergy, Karol Marcinkowski Medical Academy, Poznań, Poland.
Arch Immunol Ther Exp (Warsz). 1995;43(1):11-4.
To evaluate the effect of interleukin 10 (IL-10) on acute phase response, we determined serum levels of IL-10, interleukin 6 (IL-6), C-reactive protein (CRP), alpha-1-acid glycoprotein (AGP), alpha-1-anti-chymotrypsin (ACT) in 34 rheumatoid arthritis (RA) patients. IL-10 and IL-6 levels were evaluated using an enzyme-linked immunoassay (ELISA). CRP, AGP and ACT levels were measured using rocket immunoelectrophoresis. The results showed that IL-10 serum level was increased in RA patients as compared to controls (60.0 +/- 17.5 pg/ml vs. 7.2 +/- 1.9 pg/ml). IL-6 level was significantly elevated (87.3 +/- 32.7 pg/ml vs. 45 +/- 19 pg/ml, p < 0.05). CRP was significantly increased as compared to healthy controls (34 +/- 19 mg/1 vs. 3 +/- 2 mg/1, p < 0.05). AGP and ACT serum levels were increased in RA patients, but we did not find these changes to be statistically significant. A good negative correlation between IL-10 and IL-6 serum level was found (r = -0.73, p < 0.05). A positive significant correlation between IL-6 serum level and CRP (r = 0.62, p < 0.05), AGP (r = 0.74, p < 0.05) and ACT (r = 0.45, p < 0.05) was established. Moreover, a negative correlation between IL-10 and serum level of CRP (r = -0.76, p < 0.05), AGP (r = -0.60, p < 0.05) and ACT (r = -0.37, p < 0.05) was also shown. According to the data thus far obtained it seems that IL-10 decreases IL-6 production, and by that indirectly affects acute phase response, decreasing CRP, AGP and ACT synthesis.
为评估白细胞介素10（IL-10）对急性期反应的影响，我们测定了34例类风湿关节炎（RA）患者血清中IL-10、白细胞介素6（IL-6）、C反应蛋白（CRP）、α1-酸性糖蛋白（AGP）、α1-抗糜蛋白酶（ACT）的水平。采用酶联免疫吸附测定法（ELISA）评估IL-10和IL-6水平。使用火箭免疫电泳法测定CRP、AGP和ACT水平。结果显示，与对照组相比，RA患者血清IL-10水平升高（60.0±17.5 pg/ml对7.2±1.9 pg/ml）。IL-6水平显著升高（87.3±32.7 pg/ml对45±19 pg/ml，p<0.05）。与健康对照组相比，CRP显著升高（34±19 mg/1对3±2 mg/1，p<0.05）。RA患者血清AGP和ACT水平升高，但我们发现这些变化无统计学意义。发现IL-10与血清IL-6水平之间存在良好的负相关（r=-0.73，p<0.05）。血清IL-6水平与CRP（r=0.62，p<0.05）、AGP（r=0.74，p<0.05）和ACT（r=0.45，p<0.05）之间存在显著正相关。此外，IL-10与CRP（r=-0.76，p<0.05）、AGP（r=-0.60，p<0.05）和ACT（r=-0.37，p<0.05）的血清水平之间也呈负相关。根据目前获得的数据，似乎IL-10可降低IL-6的产生，并由此间接影响急性期反应，减少CRP、AGP和ACT的合成。
