Tisdale M J, McDevitt T M, Todorov P T, Cariuk P
Pharmaceutical Science Institute, Aston University, Birmingham, United Kingdom.
In Vivo. 1996 Mar-Apr;10(2):131-6.
A lipid mobilizing factor has been purified from a cachexia-inducing mouse colon adenocarcinoma (MAC16) using a combination of ion exchange (Mono Q), exclusion (Superose) and reverse phase hydrophobic chromatography. The purification process led to a 3,500-fold increase in the specific activity. Serum from mice bearing the MAC16 tumour contained antibodies reactive with fractions containing lipid mobilizing activity and detectable as a 24 kDa immunoreactive band on Western blotting. Serum from mice transplanted with a related tumour, MAC13, not producing cachexia, did not contain antibodies. A similar immunoreactive band was detectable in the urine of patients with cancer cachexia, but was absent from the urine of normal subjects. A monoclonal antibody produced by fusion of splenocytes from mice bearing the MAC16 tumour with mouse Balb/c myeloma cells attenuated the development of cachexia in mice transplanted with the MAC16 tumour and inhibited tumour growth. These results suggest that the M(r) 24 kDa antigen may be important in tumour growth and cachexia.
利用离子交换（Mono Q）、排阻（Superose）和反相疏水色谱相结合的方法，从一种可诱发恶病质的小鼠结肠腺癌（MAC16）中纯化出了一种脂质动员因子。纯化过程使比活性提高了3500倍。携带MAC16肿瘤的小鼠血清中含有与具有脂质动员活性的组分发生反应的抗体，在蛋白质印迹法上可检测为一条24 kDa的免疫反应条带。移植了相关肿瘤MAC13（不产生恶病质）的小鼠血清中则不含抗体。癌症恶病质患者的尿液中可检测到类似的免疫反应条带，但正常受试者尿液中没有。用携带MAC16肿瘤的小鼠脾细胞与小鼠Balb/c骨髓瘤细胞融合产生的单克隆抗体，可减轻移植了MAC16肿瘤的小鼠恶病质的发展，并抑制肿瘤生长。这些结果表明，分子量为24 kDa的抗原可能在肿瘤生长和恶病质中起重要作用。
