Rodgers J H, Crossland N O, Kline E R, Gillespie W B, Figueroa R A, Dorn P B
Department of Biology, University of Mississippi, University 38677, USA.
Ecotoxicol Environ Saf. 1996 Feb;33(1):30-7. doi: 10.1006/eesa.1996.0003.
A series of 12 outdoor model stream mesocosms was designed to evaluate the effects of chemicals and mixtures on the biota in stream ecosystems. An integrated design plan incorporated physical, chemical, and biological factors as well as the specific experimental objectives and effects parameters to be evaluated. Analysis of biological assemblages such as macroinvertebrates and periphyton in the model stream mesocosms demonstrated the presence of diverse and sensitive taxa. These model stream mesocosms also included the ability to evaluate responses of sentinel fish species such as Pimephales promelas and Lepomis macrochirus as well as macrophytes. The design and construction of the stream are discussed in detail and a brief description of a typical experimental protocol is provided. Experiments, to date, have spanned 14-30 days pretreatment "colonization" with 30 days of treatment and 15 days of posttreatment observation. Precision and accuracy of test chemical delivery to the stream have been excellent and the overall experimental design has been useful for delivering threshold toxicity concentrations and evaluating ecosystem potency for the chemicals tested.
设计了一系列12个室外模型溪流中宇宙，以评估化学物质和混合物对溪流生态系统生物群的影响。一个综合设计方案纳入了物理、化学和生物因素，以及要评估的特定实验目标和效应参数。对模型溪流中宇宙中的大型无脊椎动物和附生植物等生物群落的分析表明，存在多样且敏感的分类群。这些模型溪流中宇宙还具备评估诸如黑头软口鲦和大口黑鲈等指示鱼类物种以及大型植物反应的能力。详细讨论了溪流的设计和构建，并提供了典型实验方案的简要描述。迄今为止，实验包括14 - 30天的预处理“定殖”、30天的处理以及15天的处理后观察。测试化学物质输送到溪流中的精度和准确性极佳，总体实验设计对于确定受试化学物质的毒性阈值浓度和评估生态系统效力很有用。
