Aznar C, Lopez-Bergami P, Brandariz S, Mariette C, Liegeard P, Alves M D, Barreiro E L, Carrasco R, Lafon S, Kaplan D
Centre de Biologie Medicale Specialisee, Institut Pasteur, Paris, France.
FEMS Immunol Med Microbiol. 1995 Dec;12(3-4):231-8. doi: 10.1111/j.1574-695X.1995.tb00197.x.
Infection with Trypanosoma cruzi develops in three phases: acute, indeterminate or asymptomatic, and chronic phase (with cardiac or digestive manifestations). Moreover, transmission may occur from infected mothers to newborn, the so-called congenital form. In the present study, humoral responses against T. cruzi total extract and against the 13 amino acid peptide named R-13 derived from the parasite ribosomal P protein, previously described as a possible marker of chronic Chagas heart disease, were determined in chagasic patients and in blood bank donors from endemic areas. While in sera from acute phase, only IgM anti-T.cruzi response was observed, both IgM and IgG anti-T. cruzi antibodies were detected in sera from congenitally infected newborns. The percentage of positive response in sera from blood bank donors was relatively high in endemic regions. Antibodies against the R-13 peptide were present in a large proportion of cardiac chagasic patients but were totally lacking in patients with digestive form of Chagas' disease. Furthermore, anti-R-13 positive responses were detected in congenitally infected newborns.
急性期、不确定期或无症状期以及慢性期（伴有心脏或消化系统表现）。此外，感染的母亲可能会将病原体传播给新生儿，即所谓的先天性感染形式。在本研究中，我们检测了恰加斯病患者和来自流行地区血库捐赠者针对克氏锥虫全提取物以及针对先前被描述为慢性恰加斯心脏病可能标志物的、源自寄生虫核糖体P蛋白的名为R - 13的13氨基酸肽的体液免疫反应。在急性期血清中，仅观察到IgM抗克氏锥虫反应，而在先天性感染新生儿的血清中，同时检测到IgM和IgG抗克氏锥虫抗体。在流行地区，血库捐赠者血清中的阳性反应百分比相对较高。针对R - 13肽的抗体在大部分心脏型恰加斯病患者中存在，但在消化型恰加斯病患者中完全不存在。此外，在先天性感染新生儿中检测到抗R - 13阳性反应。
