Takeda K, Yu Z X, Nishikawa T, Tanaka M, Hosoda S, Ferrans V J, Kasajima T
Department of Pathology, Tokyo Women's Medical College, Japan.
J Mol Cell Cardiol. 1996 Jan;28(1):209-15. doi: 10.1006/jmcc.1996.0020.
Histologic, ultrastructural and nick end labeling studies were made of the process of apoptosis in the bulbus cordis of rat embryos. Apoptosis was observed between the 14th and 16th days of gestational age, at which time the bulbus cordis undergoes extensive remodeling. Three types of mesenchymal cells were identified in this region: cells undergoing apoptosis, cells engaging in the phagocytic removal of apoptotic cells, and cells not involved in either of these two processes. Fragmentation of DNA, demonstrated by nick end labeling, was found only in the apoptotic cells. The combined use of morphologic and labeling techniques is extremely useful in the evaluation of the contribution of apoptosis to cardiac morphogenesis.
对大鼠胚胎心球凋亡过程进行了组织学、超微结构和缺口末端标记研究。在孕龄第14至16天观察到凋亡，此时心球经历广泛重塑。在该区域鉴定出三种间充质细胞：正在经历凋亡的细胞、参与吞噬清除凋亡细胞的细胞以及未参与这两个过程中任何一个的细胞。缺口末端标记显示，DNA片段化仅在凋亡细胞中发现。形态学和标记技术的联合应用在评估凋亡对心脏形态发生的作用方面极为有用。
