Tranquillo R T, Girton T S, Bromberek B A, Triebes T G, Mooradian D L
Department of Chemical Engineering, University of Minnesota, Minneapolis 55455, USA.
Biomaterials. 1996 Feb;17(3):349-57. doi: 10.1016/0142-9612(96)85573-6.
Circumferential orientation of collagen fibrils in a media-equivalent (ME) is achieved in a simple and effective way using the orientating effects of a strong magnetic field during collagen fibrillogenesis when the ME is first created. Circumferential orientation of the entrapped smooth muscle cells (SMC) is achieved subsequently via cell contact guidance, the induced SMC orientation along orientated fibrils. After describing the methods used, several lines of evidence are provided showing that the magnetically orientated ME is circumferentially orientated, including collagen birefringence, circumferential SMC orientation, accelerated ME compaction and increased ME stiffness with reduced creep in the circumferential direction as compared to control MEs not exposed to a magnetic field during fibrillogenesis. The optimization of these methods is discussed in order to better mimic the circumferential orientation and mechanical properties of a natural medium. Other applications of magnetically orientated tissue-equivalents are indicated.
当首次形成介质等效物（ME）时，利用强磁场在胶原纤维形成过程中的定向作用，能够以简单有效的方式实现胶原纤维在ME中的周向排列。随后，通过细胞接触导向作用，使被困的平滑肌细胞（SMC）沿定向纤维诱导排列，从而实现周向排列。在描述所使用的方法后，提供了几条证据表明，磁定向的ME呈周向排列，包括胶原双折射、SMC周向排列、ME压实加速以及与在纤维形成过程中未暴露于磁场的对照ME相比，ME在周向方向上的刚度增加且蠕变减小。为了更好地模拟天然介质的周向排列和力学性能，对这些方法的优化进行了讨论。还指出了磁定向组织等效物的其他应用。
