Cheng G, Tu X, Dong X, Su J
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1995 Dec;35(6):450-4.
Anaerobic coculture BF2 which degraded butyrate into acetate and produced methane was isolated from granular methanogenic sludge. The coculture is associated syntrophically the Syntrophomonas subsp. saponavida strain CF2 with Methanobacterium formicicum strain MF2 and appeared to degraded C4 approximately C18 fatty acids including isobutyrate. The optimal temperature and pH for growth was 37 degrees C and 7.7 respectively. The strain CF2 was obtained in pure culture with crotonate as substrate and produces acetate and butyrate. The doubling time of strain CF2 in crotonate media was about 20 hours. Strain CF2 is Gram negative, slightly curved 0.2 approximately 0.3 x 2.0 approximately 3.0 micron with round ends, motile by lateral flagellation at the concave side, non sporeforming. With a hydrogen scavenging organism, such as Methanospillum hungatei JF1, Methanobacterium formicicum 1535, Methanobrevibacterium bryantii 1125 and Desulfovibrio sp. B11, the strain CF2 paired up and the defined coculture degraded butyrate to acetate. When the strain CF2 associated with the original accompanist, Methanobacterium formicius strain MF2, the reassociated couculture degraded butyrate to acetate and produced methane again.
从颗粒状产甲烷污泥中分离出了厌氧共培养物BF2，它能将丁酸盐降解为乙酸盐并产生甲烷。该共培养物与嗜互营单胞菌属皂化嗜互营单胞菌菌株CF2和甲酸甲烷杆菌菌株MF2进行共生关联，似乎能降解包括异丁酸盐在内的C4至C18脂肪酸。生长的最佳温度和pH分别为37℃和7.7。菌株CF2以巴豆酸盐为底物进行纯培养，产生乙酸盐和丁酸盐。菌株CF2在巴豆酸盐培养基中的倍增时间约为20小时。菌株CF2为革兰氏阴性菌，略微弯曲，0.2至0.3×2.0至3.0微米，两端圆形，在凹面通过侧生鞭毛运动，不形成芽孢。与诸如亨氏甲烷溢菌JF1、甲酸甲烷杆菌1535、布氏甲烷短杆菌1125和脱硫弧菌属B11等氢清除生物体一起时，菌株CF2配对，确定的共培养物将丁酸盐降解为乙酸盐。当菌株CF2与原始伴生菌甲酸甲烷杆菌菌株MF2关联时，重新关联的共培养物再次将丁酸盐降解为乙酸盐并产生甲烷。
