Fedorenko G M, Gusatinsky V N, Kaminsky I I, Kondratyeva L A, Korzak V M
A. B. Kogan Research Institute for Neurocybernetics, Rostov-on-Don, Russia.
Neuroreport. 1995 Nov 27;6(17):2325-32. doi: 10.1097/00001756-199511270-00014.
We investigated the influence of prolonged excitation or combined excitation/inhibition on the ultrastructural changes in an isolated stretch receptor neurone from crayfish abdomen. Excitatory impulse activity was achieved by mechanical stimulation of a neurone for 10 or 120 min and inhibition of the activity was obtained by perfusion with gamma-aminobutyric acid (GABA, 2.5 x 10(-5) M) for the same periods. Changes in the ultrastructural components were observed following both excitatory and inhibitory stimulation for 120 min. Neuronal adaptation to excitatory or inhibitory stimuli after 120 min resulted in the appearance of an increased number of the giant mitochondria. Thus, the number of giant mitochondria may serve as an ultrastructural index of the degree of neuronal adaptation to external stimulation.
我们研究了长时间兴奋或联合兴奋/抑制对小龙虾腹部分离的牵张感受器神经元超微结构变化的影响。通过对神经元进行10或120分钟的机械刺激来实现兴奋性冲动活动，通过在相同时间段内灌注γ-氨基丁酸（GABA，2.5×10⁻⁵ M）来抑制活动。在兴奋性和抑制性刺激120分钟后观察超微结构成分的变化。120分钟后神经元对兴奋性或抑制性刺激的适应导致巨型线粒体数量增加。因此，巨型线粒体的数量可作为神经元对外界刺激适应程度的超微结构指标。
