Elekes K, Florey E
Fakultät für Biologie der Universität Konstanz, F.R.G.
Neuroscience. 1987 Sep;22(3):1111-22. doi: 10.1016/0306-4522(87)92986-1.
The GABAergic innervation of the stretch receptor neurons of the crayfish Orconectes limosus has been investigated by means of light- and electron microscope immunocytochemistry using an antibody to GABA. Both whole-mount preparations and post-embedding semithin sections revealed a massive GABAergic innervation of both the slowly and the fast adapting receptor neurons. The stretch receptor organ is supplied by one principle GABA-immunoreactive axon, which gives off several branches that innervate the receptor neurons. Cell body, initial axon segment and dendritic region of the sensory neurons are covered by numerous GABA-immunoreactive varicose fibers. Electron microscopy revealed that the GABA-immunoreactive varicosities establish specialized synaptic contacts with the sensory neurons. The functional significance of the occurrence of GABA-immunoreactive varicosities on the different parts of the sensory neurons is discussed. The results support the physiological and pharmacological evidence that GABA is a transmitter substance of the efferent inhibitory neurons which innervate the crayfish stretch receptor neurons.
利用抗γ-氨基丁酸（GABA）抗体，通过光学显微镜和电子显微镜免疫细胞化学方法，对小龙虾奥氏原螯虾（Orconectes limosus）牵张感受器神经元的GABA能神经支配进行了研究。整装标本和包埋后半薄切片均显示，慢适应和快适应感受器神经元均有大量的GABA能神经支配。牵张感受器器官由一条主要的GABA免疫反应性轴突供应，该轴突发出几条分支，支配感受器神经元。感觉神经元的胞体、轴突起始段和树突区域被大量GABA免疫反应性曲张纤维覆盖。电子显微镜显示，GABA免疫反应性曲张体与感觉神经元建立了特殊的突触联系。文中讨论了GABA免疫反应性曲张体在感觉神经元不同部位出现的功能意义。这些结果支持了生理学和药理学证据，即GABA是支配小龙虾牵张感受器神经元的传出抑制性神经元的递质。
