Vértes Z, Környei J L, Kovács S, Vértes M
Institute of Physiology, University Medical School, Pécs, Hungary.
Neuroreport. 1995 Nov 27;6(17):2385-8. doi: 10.1097/00001756-199511270-00026.
High affinity (Kd approximately 1 nM) and low capacity [3H]naloxone binding sites were detected in the nuclear fraction of rat hypothalamus. The profile of this binding changed with age. In immature rats from 11 days of age until vaginal opening (approximately 33 day) the Scatchard plots of saturation data were linear and the Hill coefficient was 1, while just after vaginal opening (< 6 h) Scatchard analysis of [3H]naloxone binding gave a curvilinear component, with the Hill coefficient approximately 2, followed by an increase in binding sites and a decrease in Kd. In adults, after ovariectomy, the pattern of [3H]naloxone binding was similar to that observed in immature rats before vaginal opening. Following oestradiol treatment, binding sites with linear Scatchard plots disappeared and returned at least 24 h after hormone administration.
在大鼠下丘脑的核部分检测到高亲和力（解离常数约为1 nM）和低容量的[3H]纳洛酮结合位点。这种结合的特征随年龄而变化。在11日龄至阴道开口（约33日龄）的未成熟大鼠中，饱和数据的Scatchard图呈线性，希尔系数为1，而在阴道开口后不久（<6小时），[3H]纳洛酮结合的Scatchard分析给出了一个曲线成分，希尔系数约为2，随后结合位点增加，解离常数降低。在成年大鼠中，卵巢切除术后，[3H]纳洛酮结合模式与阴道开口前未成熟大鼠中观察到的相似。雌二醇治疗后，具有线性Scatchard图的结合位点消失，并在激素给药后至少24小时恢复。
