Bahner U, Geiger H, Palkovits M, Luft F C, Heidland A
Department of Internal Medicine, University of Wuerzburg, FRG.
Hypertens Res. 1995 Dec;18(4):279-84. doi: 10.1291/hypres.18.279.
Angiotensin (Ang) II and atrial natriuretic peptide (ANP) have opposing effects on blood pressure, sympathetic activity, vasopressin and ACTH secretion, salt appetite, and drinking. We observed their interaction by infusing Ang II (7.2 nmol/h) into the peritoneum (i.p.) or into the lateral ventricle (i.c.v.) of rats with osmotic minipumps for seven days. At sacrifice, rats receiving Ang II-i.c.v. had a systolic blood pressure of 184 +/- 3 (SEM) mmHg, those receiving Ang II-i.p. had 159 +/- 5 mmHg (p < 0.05), while controls had 109 +/- 2 and 110 +/- 2 mmHg, respectively (p < 0.05). Drinking and urine volume increased similarly in rats receiving Ang II by either route, while Uosm decreased. Renin (PRA) values were lower (p < 0.05) in rats receiving Ang II-i.c.v. (0.7 +/- 0.2 ng Ang l/ml/h) or Ang II-i.p. (0.9 +/- 0.2) than in the respective controls (2.3 +/- 0.7 and 2.0 +/- 0.3). Plasma ANP values with Ang II-i.c.v. (18 +/- 1.6 pg/ml) or with Ang II-i.p. (49 +/- 6) were also lower (p < 0.05) than respective controls (89 +/- 12, 76 +/- 4). Vasopressin (AVP) concentrations in the plasma were not influenced by the regimens. In the brain, the ANP contents in areas of the so-called AV3V-region (organum vasculosum laminae terminalis, preoptic periventricular nucleus, medial preoptic nucleus) were similarly and significantly reduced by both Ang II-i.c.v. and Ang II-i.p.. ANP values were also reduced in the median eminence by both types of Ang II-treatment, while ANP concentrations in the supraoptic nucleus were increased. The data show that Ang II infusions producing a chronic rise in blood pressure exert similar effects on drinking behavior, PRA, and ANP concentrations in blood and brain. The AV3V area may be pivotal to both models.
血管紧张素（Ang）II和心房利钠肽（ANP）对血压、交感神经活动、血管加压素和促肾上腺皮质激素分泌、盐食欲及饮水具有相反的作用。我们通过用渗透微型泵向大鼠腹膜内（i.p.）或侧脑室内（i.c.v.）输注Ang II（7.2 nmol/h）7天来观察它们之间的相互作用。处死时，接受i.c.v.途径Ang II输注的大鼠收缩压为184±3（SEM）mmHg，接受i.p.途径Ang II输注的大鼠收缩压为159±5 mmHg（p<0.05），而对照组分别为109±2和110±2 mmHg（p<0.05）。两种途径接受Ang II输注的大鼠饮水和尿量均同样增加，而尿渗透压降低。接受i.c.v.途径Ang II输注（0.7±0.2 ng Ang I/ml/h）或i.p.途径Ang II输注（0.9±0.2）的大鼠肾素（PRA）值低于各自对照组（2.3±0.7和2.0±0.3）（p<0.05）。i.c.v.途径Ang II输注（18±1.6 pg/ml）或i.p.途径Ang II输注（49±6）时的血浆ANP值也低于各自对照组（89±12，76±4）（p<0.05）。血浆中血管加压素（AVP）浓度不受这些给药方案的影响。在脑内，所谓AV3V区（终板血管器、视前室周核、视前内侧核）区域的ANP含量经i.c.v.和i.p.途径的Ang II输注均同样显著降低。两种类型的Ang II处理均使正中隆起处的ANP值降低，而视上核中的ANP浓度升高。数据表明，使血压长期升高的Ang II输注对饮水行为、PRA以及血液和脑内的ANP浓度产生相似的影响。AV3V区可能在两种模型中都起关键作用。
