Feng M, van der Does L, Bantjes A
Department of Chemical Technology, Biomaterials Section, University of Twente, Enschede, The Netherlands.
J Dairy Sci. 1995 Nov;78(11):2352-7. doi: 10.3168/jds.S0022-0302(95)76862-X.
Iron(III) removal from lactoferrin by an iron(III)-chelating resin with immobilized 3-hydroxy-2-methyl-4(1H)-pyridinone ligands was studied at physiological pH in the presence of citrate. The resin had a marked effect on the extent of iron removal. By using the iron(III)-chelating resin, removal of iron from lactoferrin was nearly complete in < 24 h. Apolactoferrin with 4% iron saturation could be prepared conveniently from 100% or from 18% iron-saturated lactoferrin under mild conditions without affecting the iron-binding capacity of the protein. The iron saturation of the obtained apolactoferrin was much lower than that of the apolactoferrin prepared by reported methods.
在生理pH值和柠檬酸盐存在的条件下，研究了用固定有3-羟基-2-甲基-4(1H)-吡啶酮配体的铁(III)螯合树脂从乳铁蛋白中去除铁(III)的情况。该树脂对铁的去除程度有显著影响。通过使用铁(III)螯合树脂，在不到24小时内乳铁蛋白中的铁几乎被完全去除。在温和条件下，可以方便地从100%或18%铁饱和的乳铁蛋白制备铁饱和度为4%的脱铁乳铁蛋白，且不会影响该蛋白质的铁结合能力。所获得的脱铁乳铁蛋白的铁饱和度远低于通过报道方法制备的脱铁乳铁蛋白。
