Kleber J W, Galloway J A, Rodda B E
J Pharm Sci. 1977 May;66(5):635-8. doi: 10.1002/jps.2600660507.
A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method.
已开发出一种灵敏且特异的气相色谱（GLC）分析法，用于测定血浆和尿液中的醋磺己脲及其主要代谢物羟基己脲。该分析法使用甲苯磺丁脲作为质量内标。化合物从酸化的血浆或尿液中用甲苯萃取，用硫酸二甲酯转化为甲基化衍生物，并用带有火焰离子化检测器的气相色谱法进行测定。通过气相色谱 - 质谱联用，所测定的化合物是气相色谱热解产生的N - 甲基磺酰胺。本文给出了一项生物利用度研究中的血浆和尿液数据，以证明该方法的实用性。
