Vinayak R, Andrus A, Sinha N D, Hampel A
Department of Biological Sciences, Northern Illinois University, DeKalb 60115, USA.
Anal Biochem. 1995 Dec 10;232(2):204-9. doi: 10.1006/abio.1995.0008.
An HPLC procedure has been developed that can be used to monitor the rate of phosphodiester cleavage of an oligoribonucleotide substrate by an RNA ribozyme with catalytic activity. The method operates at high substrate concentrations (far beyond K(m)), thus allowing the reactions to approach Vmax of the reaction. This method is an efficient alternative to radioisotope labeling methods.
