Assay of ribozyme-substrate cleavage by anion-exchange high-performance liquid chromatography.
作者信息
Vinayak R, Andrus A, Sinha N D, Hampel A
机构信息
Department of Biological Sciences, Northern Illinois University, DeKalb 60115, USA.
出版信息
Anal Biochem. 1995 Dec 10;232(2):204-9. doi: 10.1006/abio.1995.0008.
PMID:8747476
Abstract
An HPLC procedure has been developed that can be used to monitor the rate of phosphodiester cleavage of an oligoribonucleotide substrate by an RNA ribozyme with catalytic activity. The method operates at high substrate concentrations (far beyond K(m)), thus allowing the reactions to approach Vmax of the reaction. This method is an efficient alternative to radioisotope labeling methods.
摘要
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