Mohan N, Sadeghi K, Reiter R J, Meltz M L
Department of Radiology, University of Texas Health Science Center, San Antonio 78284-7800, USA.
Biochem Mol Biol Int. 1995 Dec;37(6):1063-70.
Recently, the neurohormone melatonin was found to be a potent endogenous hydroxyl radical scavenger. Since the activation of an important transcriptional regulator, nuclear factor-kappa B (NF-kappa B) involves free radicals as second messengers, we investigated whether melatonin alters NF-kappa B expression if present during treatment of HeLa S3 cells with known activators. The results show that upon treatment of HeLa S3 cells with tumor necrosis factor-alpha or phorbol 12-myristate 13-acetate, or with ionizing radiation, there is a profound induction of NF-kappa B binding activity. If present at the time of treatment, exogenously added melatonin, at a concentration as low as 10 microM, was found to inhibit the activation of NF-kappa B by these agents. This results leads to the hypothesis that melatonin can play a role in many physiological functions through its modulation of NF-kappa B (and possibly other) transcriptional regulators.
最近，人们发现神经激素褪黑素是一种强大的内源性羟自由基清除剂。由于重要的转录调节因子核因子-κB（NF-κB）的激活涉及自由基作为第二信使，我们研究了在使用已知激活剂处理HeLa S3细胞期间，如果存在褪黑素，它是否会改变NF-κB的表达。结果表明，在用肿瘤坏死因子-α或佛波酯12-肉豆蔻酸酯13-乙酸酯处理HeLa S3细胞时，或在进行电离辐射时，NF-κB结合活性会有显著诱导。如果在处理时存在，发现外源性添加的褪黑素，浓度低至10微摩尔，可抑制这些试剂对NF-κB的激活。这一结果引出了一个假设，即褪黑素可以通过调节NF-κB（以及可能的其他）转录调节因子在许多生理功能中发挥作用。
