Kouyanou S, Pilali M, Fragoulis E G
Department of Biochemistry, Cell and Molecular Biology and Genetics, University of Athens, Greece.
Biochem Mol Biol Int. 1995 Dec;37(6):1217-27.
A 34 kDa, poly(U) and poly(C)-specific ribonuclease, is shown to be tightly bound on purified polysomes from six-day-old larvae of the insect Ceratitis capitata. High salt treatment (400 mM KCl) is necessary to release it completely from the polysomes. Removal of the RNase does not disrupt the structure of the ribosomes, as shown by centrifugation on sucrose gradients and poly U directed polyphenylalanine synthesis.
一种分子量为34 kDa、对聚尿苷酸（poly(U)）和聚胞苷酸（poly(C)）具有特异性的核糖核酸酶，被证明紧密结合在果蝇实蝇六日龄幼虫的纯化多核糖体上。需要用高盐处理（400 mM KCl）才能将其从多核糖体上完全释放出来。如在蔗糖梯度上离心和聚尿苷酸指导的聚苯丙氨酸合成所示，去除核糖核酸酶不会破坏核糖体的结构。
