血小板反应的遗传学。II. HLA - DRw是主要的血小板刺激决定因素。

The genetics of PLT response. II. HLA-DRw is a major PLT-stimulating determinant.

作者信息

DeWolf W C, Carroll P G, Mehta C R, Martin S L, Yunis E J

出版信息

J Immunol. 1979 Jul;123(1):37-42.

Abstract

PLT response is restricted by the HLA-D region. The present study was undertaken to help define the role of HLA-DRw in PLT restimulation. Haplotype-primed intrafamily PLT cells were made against specificities HLA-DRw1, HLA-DRw3, and HLA-DRw7; each PLT was then restimulated with cells from a 35-member unrelated panel. Restimulation values for each PLT were subjected to bimodal clustering analysis. In addition, blocking experiments were performed with other intrafamily and homozygous typing cell PLT after preincubation with B cell alloantisera. The results show a high correlation (0.881 less than or equal to r less than or equal to 1.00) between the HLA-DRw specificity of the priming haplotype and the HLA-DRw specificity of unrelated panel cells that restimulate in PLT. When stimulating cells were absorbed with the corresponding DRw alloantisera or p29,34 heteroantiserum (against B cell specific antigens), PLT restimulation was significantly blocked. However, the PLT cells treated with antisera showed no effect. The results strongly suggest that HLA-DRw is the principal PLT-stimulating determinant.

摘要

血小板（PLT）反应受HLA - D区域限制。本研究旨在帮助确定HLA - DRw在血小板再刺激中的作用。制备针对HLA - DRw1、HLA - DRw3和HLA - DRw7特异性的单倍型致敏家族内血小板细胞；然后用来自一个35名无关个体的细胞对每个血小板进行再刺激。对每个血小板的再刺激值进行双峰聚类分析。此外，在用B细胞同种异体抗血清预孵育后，对其他家族内和纯合分型细胞血小板进行阻断实验。结果显示，致敏单倍型的HLA - DRw特异性与在血小板中进行再刺激的无关个体细胞的HLA - DRw特异性之间存在高度相关性（0.881≤r≤1.00）。当刺激细胞用相应的DRw同种异体抗血清或p29,34异种抗血清（针对B细胞特异性抗原）吸收时，血小板再刺激被显著阻断。然而，用抗血清处理过的血小板细胞没有效果。结果强烈表明，HLA - DRw是主要的血小板刺激决定因素。