Mutugi M W, Boid R, Luckins A G
Kenya Trypanosomiasis Research Institute (KETRI), Kikuyu, Kenya.
Vet Parasitol. 1995 Dec;60(3-4):213-20. doi: 10.1016/0304-4017(95)00787-4.
Four Trypanosoma evansi stocks with sensitivity to suramin in mice ranging from 0.05 to 160 mg kg-1 were cloned and sub-cloned and the sensitivity of the clones determined. The results suggest that it is easier to clone and sub-clone trypanosome stocks which are sensitive to suramin than those that are resistant to the action of the drug. The clones obtained from the four stocks had sensitivities to suramin which were similar to or different from the parent stocks. These results are important in view of the development of resistance for, in the presence of suramin, these resistant yet heterogeneous populations would provide the material from which selective processes could operate. These observations also suggest that the maintenance and spread of suramin-resistant trypanosomes might be curtailed by their comparative inability to establish themselves in a new host.
对苏拉明敏感的4株伊氏锥虫原种，在小鼠体内的敏感性范围为0.05至160毫克/千克，对其进行克隆和亚克隆，并测定克隆株的敏感性。结果表明，与对该药物作用具有抗性的锥虫原种相比，对苏拉明敏感的锥虫原种更容易进行克隆和亚克隆。从这4个原种获得的克隆株对苏拉明的敏感性与亲代原种相似或不同。鉴于耐药性的发展，这些结果很重要，因为在苏拉明存在的情况下，这些具有抗性但异质的群体将提供可供选择过程起作用的材料。这些观察结果还表明，对苏拉明耐药的锥虫在新宿主中相对难以定殖，这可能会限制其维持和传播。
