al-Shamma H A, De Vries G J
Neuroscience and Behavior Program, University of Massachusetts, Amherst 01003-7710, USA.
J Neurobiol. 1996 Jan;29(1):91-8. doi: 10.1002/(SICI)1097-4695(199601)29:1<91::AID-NEU7>3.0.CO;2-2.
The bed nucleus of the stria terminalis (BNST) and centromedial amygdala share many neuroantomical and neurochemical characteristics, suggesting similarities in their development. Here we compare the neurogenesis of a group of cells for which already several common characteristics have been documented, that is, the sexually dimorphic arginine vasopressin-immunoreactive (AVP-ir) cells of the BNST and amygdala. To determine when these cells are born, pregnant rats received intraperitoneal injections of the thymidine analogue bromo-2-deoxy-5-uridine (BrdU) on one of nine embryonic days, E10 to E18; E1 being the day that a copulatory plug was found. At 3 months of age, the offsprings of these females were killed and their brains stained immunocytochemically for BrdU and AVP. Most AVP-ir cells were labeled with BrdU by injections on E12 and E13. Although BrdU labeling of AVP-ir cells did not differ between the BNST and amygdala, it differed between males and females. From E12 to E13, the percentage of BrdU-labeled AVP-ir cells decreased more in males than in females. AVP-ir cells appeared to be born earlier than most other cells in the same area, the majority of which were labeled with BrdU by injections on E14, E15, and E16. The similarities in the birthdates of AVP-ir cells in the BNST and amygdala may help to explain why these cells take on so many similar characteristics. The sex difference in birthdates of AVP-ir cells may help to explain which cellular processes underlie the sexual differentiation of these cells.
终纹床核（BNST）和杏仁核中央内侧核具有许多神经解剖学和神经化学特征，表明它们在发育过程中存在相似性。在此，我们比较了一组已被记录有若干共同特征的细胞的神经发生情况，即BNST和杏仁核中具有性别差异的精氨酸加压素免疫反应性（AVP-ir）细胞。为了确定这些细胞的诞生时间，怀孕大鼠在胚胎期的九个日子（E10至E18）中的一天接受腹腔注射胸腺嘧啶类似物溴脱氧尿苷（BrdU）；E1为发现交配栓的日子。在3个月大时，处死这些雌性大鼠的后代，并对其大脑进行免疫细胞化学染色，以检测BrdU和AVP。大多数AVP-ir细胞在E12和E13注射时被BrdU标记。虽然BNST和杏仁核中AVP-ir细胞的BrdU标记没有差异，但在雄性和雌性之间存在差异。从E12到E13，雄性中BrdU标记的AVP-ir细胞百分比下降幅度大于雌性。AVP-ir细胞似乎比同一区域的大多数其他细胞诞生得更早，同一区域的大多数其他细胞在E14、E15和E16注射时被BrdU标记。BNST和杏仁核中AVP-ir细胞出生日期的相似性可能有助于解释为什么这些细胞具有如此多相似的特征。AVP-ir细胞出生日期的性别差异可能有助于解释这些细胞性别分化背后的细胞过程。
