Otero-Garcia Marcos, Martin-Sanchez Ana, Fortes-Marco Lluis, Martínez-Ricós Joana, Agustin-Pavón Carmen, Lanuza Enrique, Martínez-García Fernando
Laboratori de Neuroanatomia Funcional Comparada, Depts. Biologia Funcional i Biologia Cel·lular, Fac. Ciències Biològiques, Univ. València, C. Dr. Moliner, 50, 46100, Burjassot, Spain.
Brain Struct Funct. 2014 May;219(3):1055-81. doi: 10.1007/s00429-013-0553-3. Epub 2013 Apr 30.
Quantitative analysis of the immunoreactivity for arginine-vasopressin (AVP-ir) in the telencephalon of male (intact and castrated) and female CD1 mice allows us to precisely locate two sexually dimorphic (more abundant in intact than castrated males and females) AVP-ir cell groups in the posterior bed nucleus of the stria terminalis (BST) and the amygdala. Chemoarchitecture (NADPH diaphorase) reveals that the intraamygdaloid AVP-ir cells are located in the intra-amygdaloid BST (BSTIA) rather than the medial amygdala (Me), as previously thought. Then, we have used for the first time tract tracing (combined with AVP immunofluorescence) and fiber-sparing lesions of the BST to analyze the projections of the telencephalic AVP-ir cell groups. The results demonstrate that the posterior BST originates the sexually dimorphic innervation of the lateral septum, the posterodorsal Me and a substance P-negative area in the medioventral striato-pallidum (mvStP).The BSTIA may also contribute to some of these terminal fields. Our material also reveals non-dimorphic AVP-ir processes in two locations of the amygdala. First, the ventral Me shows dendrite-like AVP-ir processes apparently belonging supraoptic neurons, whose possible functions are discussed. Second, the Ce shows sparse, thick AVP-ir axons with high individual variability in density and distribution, whose possible influence on stress coping in relation to the affiliative or agonistic behaviors mediated by the Me are discussed. Finally, we propose that the region of the mvStP showing sexually dimorphic AVP-ir innervation is part of the brain network for socio-sexual behavior, in which it would mediate motivational aspects of chemosensory-guided social interactions.
对雄性（完整和阉割）和雌性CD1小鼠端脑内精氨酸加压素免疫反应性（AVP-ir）进行定量分析，使我们能够精确地在终纹床核后部（BST）和杏仁核中定位两个性二态性的（完整雄性和雌性比阉割雄性和雌性中更丰富）AVP-ir细胞群。化学结构（NADPH双氢酶）显示，杏仁核内的AVP-ir细胞位于杏仁核内的BST（BSTIA）而非如先前认为的内侧杏仁核（Me）。然后，我们首次使用了束路追踪（结合AVP免疫荧光）和BST的纤维保留性损伤来分析端脑AVP-ir细胞群的投射。结果表明，BST后部发出对外侧隔、后背部Me以及中腹侧纹状体苍白球（mvStP）中一个P物质阴性区域的性二态性神经支配。BSTIA可能也对其中一些终末区域有贡献。我们的材料还揭示了杏仁核两个位置存在非二态性的AVP-ir突起。首先，腹侧Me显示出类似树突的AVP-ir突起，显然属于视上核神经元，讨论了其可能的功能。其次，中央杏仁核（Ce）显示出稀疏、粗大的AVP-ir轴突，其密度和分布个体差异很大，讨论了其对与Me介导的亲和或争斗行为相关的应激应对的可能影响。最后，我们提出，显示性二态性AVP-ir神经支配的mvStP区域是社会性行为脑网络中的一部分，在其中它将介导化学感觉引导的社会互动的动机方面。
