澳大利亚断奶仔猪大肠杆菌菌株中F107菌毛的流行情况及其与志贺样毒素II的关联。

The prevalence of F107 fimbriae and their association with Shiga-like toxin II in Escherichia coli strains from weaned Australian pigs.

作者信息

Hide E J, Connaughton I D, Driesen S J, Hasse D, Monckton R P, Sammons N G

机构信息

Ausvac Pty Ltd, Bendigo, Australia.

出版信息

Vet Microbiol. 1995 Dec;47(3-4):235-43. doi: 10.1016/0378-1135(95)00117-4.

DOI:10.1016/0378-1135(95)00117-4

PMID:8748539

Abstract

A total of 480 haemolytic Escherichia coli (HEC) strains from weaned pigs were tested using an oligonucleotide probe to determine the prevalence of F107 fimbriae in Australia. Of these, 62% were positive. F107 was detected in serogroups O141, O138, O8, O45, O139, O157 and O98 but not in O149 nor O147. 81% of E. coli strains not producing other fimbriae (K88, 987P, K99 or F41) were positive for F107; 5% of strains with K88 fimbriae also had F107. Serological investigation of the expression of F107 fimbriae indicated that serogroups O141, O138, O8, O45 and O157 produced variant F107ac. Variant F107ab was found on O139 strains only. The F107 fimbrial subunits of both variants had a molecular weight of approximately 16 kDa. A total of 350 of the HEC strains were tested to determine the prevalence of the Shiga-like toxin II (SLT II) gene. 29.0% of these strains were positive. SLT II was detected in serogroups O141, O138, O149, O98, O45, O8 and O157 but not in O139. 25% of these strains were positive for both F107 and SLT II.

摘要

使用寡核苷酸探针检测了来自断奶仔猪的480株溶血性大肠杆菌（HEC）菌株，以确定澳大利亚F107菌毛的流行情况。其中，62%呈阳性。在O141、O138、O8、O45、O139、O157和O98血清群中检测到F107，但在O149和O147血清群中未检测到。81%不产生其他菌毛（K88、987P、K99或F41）的大肠杆菌菌株F107呈阳性；5%具有K88菌毛的菌株也有F107。对F107菌毛表达的血清学研究表明，O141、O138、O8、O45和O157血清群产生变异型F107ac。仅在O139菌株上发现变异型F107ab。两种变异型的F107菌毛亚基分子量约为16 kDa。检测了350株HEC菌株以确定志贺样毒素II（SLT II）基因的流行情况。这些菌株中有29.0%呈阳性。在O141、O138、O149、O98、O45、O8和O157血清群中检测到SLT II，但在O139血清群中未检测到。这些菌株中有25%的F107和SLT II均呈阳性。