Poumarat F, Solsona M
Centre National d'Etudes Vétérinaires et Alimentaires-Laboratoire de Pathologie Bovine, Lyon, France.
Vet Microbiol. 1995 Dec;47(3-4):305-15. doi: 10.1016/0378-1135(95)00115-8.
Restriction endonuclease analysis (REA) and immunoblotting were used to compare field strains of Mycoplasma mycoides subsp. mycoides biotype Small Colony from different parts of Europe and Africa. Of the 27 strains tested by REA, the European ones (n = 15), except 3 from the Iberian peninsula, differed strikingly from the African ones (n = 10). Vaccinal strains T1-SR and KH3J had a unique REA pattern. Polyclonal immunoblot profiles of 46 strains appeared fairly similar. However most of the Italian strains (22 out of 24) shared a characteristic, in the 100 kDa area, unlike that of the other European strains. Immunoprofiles of the reference strain PG1 and vaccinal strain T1-SR were different from the others. These results allowed the determination of possible links between different geographic region of enzootic contagious bovine pleuropneumonia.
使用限制性内切酶分析（REA）和免疫印迹法比较了来自欧洲和非洲不同地区的丝状支原体丝状亚种小菌落生物型的田间菌株。在通过REA测试的27株菌株中，欧洲菌株（n = 15），除了来自伊比利亚半岛的3株外，与非洲菌株（n = 10）有显著差异。疫苗株T1-SR和KH3J具有独特的REA模式。46株菌株的多克隆免疫印迹图谱相当相似。然而，大多数意大利菌株（24株中的22株）在100 kDa区域具有不同于其他欧洲菌株的特征。参考菌株PG1和疫苗株T1-SR的免疫图谱与其他菌株不同。这些结果有助于确定地方性传染性牛胸膜肺炎不同地理区域之间的可能联系。
