Thiaucourt F, Lorenzon S, David A, Tulasne J J, Domenech J
CIRAD-EMVT, Montpellier, France.
Ann N Y Acad Sci. 1998 Jun 29;849:146-51. doi: 10.1111/j.1749-6632.1998.tb11043.x.
Contagious bovine pleuropneumonia is a serious threat to cattle not only in Africa but also in southern Europe and possibly Asia. It is now present in countries that had been free of the disease for many years, giving rise to doubts about the efficiency of the control strategies. In Africa CBPP is controlled mainly by a vaccination policy that uses variant strains of Mycoplasma mycoides subsp mycoides biotype SC, called T1/44 or T1sr. Until recently, it was not possible to differentiate the various strains within the biotype and consequently to identify the vaccine strains. Restriction analysis of mycoplasma DNA with HindIII and Pst1 has been applied to 24 strains of African origin and one European strain. Each enzyme gave rise to different restriction profiles and the combination of the results permitted subdivision of these strains into 9 groups. Interestingly, some profiles of pathogenic strains seem to be restricted to certain geographical areas. The profile of the poorly immunogenic vaccinal strain KH3J is also very peculiar, and it is easily distinguished from that of the other vaccine strains originating from T1. This technique is simple once the strains are isolated. Efforts are now under way to use molecular tools based on PCR products to alleviate the difficulty of isolation.
牛传染性胸膜肺炎不仅对非洲的牛，而且对南欧乃至可能对亚洲的牛都构成严重威胁。现在，该病出现在许多年未曾出现过这种疾病的国家，这引发了人们对控制策略有效性的质疑。在非洲，牛传染性胸膜肺炎主要通过使用丝状支原体丝状亚种生物型SC的变异株（称为T1/44或T1sr）进行疫苗接种策略来控制。直到最近，还无法区分生物型内的各种菌株，因此也无法识别疫苗菌株。用HindIII和Pst1对支原体DNA进行限制性分析已应用于24株非洲来源的菌株和1株欧洲菌株。每种酶产生不同的限制性图谱，结果的组合允许将这些菌株细分为9组。有趣的是，一些致病菌株的图谱似乎局限于某些地理区域。免疫原性差的疫苗株KH3J的图谱也非常独特，很容易与源自T1的其他疫苗株区分开来。一旦分离出菌株，这项技术就很简单。目前正在努力使用基于PCR产物的分子工具来缓解分离的困难。
