Zatta P, Zambenedetti P, Marturano M B, Palumbo M, Nicolini M
Centro CNR Metalloproteine, Universita' di Padova, Italy.
J Neural Transm Gen Sect. 1995;102(2):113-23. doi: 10.1007/BF01276507.
Tacrine [9-amino-1,2,3,4-tetrahydroacridine] (THA), a potent acetylcholinesterasic inhibitor, is utilized in the pharmacological treatment of Alzheimer's disease (Birne and Arie, 1994). Cytopharmacology of THA is still largely to be discovered. In the present paper we report some effects produced by THA on murine neuroblastoma cells (N2A) used as an experimental model. N2A cells treated with THA at low concentration (1 mu M) showed a reduced cell's mitosis and a remarkable reduction of protein synthesis. Eventually, a marked reduction on the phosphorylation of proteins associated to neurofilaments 200 kD, is observed using specific antibody.
他克林9-氨基-1,2,3,4-四氢吖啶是一种有效的乙酰胆碱酯酶抑制剂，用于阿尔茨海默病的药物治疗(Birne和Arie，1994)。THA的细胞药理学仍有待大量探索。在本文中，我们报告了THA对用作实验模型的小鼠神经母细胞瘤细胞(N2A)产生的一些影响。用低浓度(1μM)的THA处理的N2A细胞显示细胞有丝分裂减少，蛋白质合成显著降低。最终，使用特异性抗体观察到与200 kD神经丝相关的蛋白质磷酸化明显减少。
