Chène C, Fountoulakis M, Döbeli H, D'Arcy B, Winkler F, D'Arcy A
Department of Pharmaceutical Research-New Technologies, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Proteins. 1995 Dec;23(4):591-4. doi: 10.1002/prot.340230415.
A complex of human interferon-gamma (IFN-gamma) with the soluble extracellular domain of the IFN-gamma receptor alpha-chain (IFN-gamma-R) has been crystallised. Crystals of the complex were grown using PEG 4000 as the precipitating agent in the presence of beta-octyl glucoside. The receptor-ligand complex crystallizes in a monoclinic space group and diffracts to about 3.0 A resolution. Isomorphous crystals have been obtained with complex containing selenomethionine and cysteine mutants of IFN-gamma, which may facilitate the ongoing X-ray structure determination.
人γ干扰素（IFN-γ）与γ干扰素受体α链（IFN-γ-R）的可溶性细胞外结构域形成的复合物已被结晶。在β-辛基葡糖苷存在的情况下，使用聚乙二醇4000（PEG 4000）作为沉淀剂培养该复合物的晶体。该受体-配体复合物在单斜晶系空间群中结晶，衍射分辨率约为3.0埃。已经获得了含有硒代蛋氨酸和IFN-γ半胱氨酸突变体的复合物的同晶型晶体，这可能有助于正在进行的X射线结构测定。
