鸡γ干扰素受体α链的蛋白质表达、结晶及初步X射线晶体学分析
Protein expression, crystallization and preliminary X-ray crystallographic analysis of chicken interferon-γ receptor α chain.
作者信息
Ping Zhiguang, Shi Yi, Sun Yanling, Ma Liping, Wang Ming
机构信息
College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):41-4. doi: 10.1107/S1744309111046318. Epub 2011 Dec 24.
Abstract
The activity of interferon-γ (IFN-γ) relies on signal transduction, which is triggered by combination with the receptors interferon-γ receptor α chain (IFNGR1) and β chain (IFNGR2). Native recombinant chicken IFNGR1 (chIFNGR1; residues 25-237) was overexpressed in Escherichia coli, purified by refolding and crystallized using the vapour-diffusion technique. The crystals belonged to space group P6(5)22, with unit-cell parameters a = b = 64.1, c = 216.3 Å, α = β = 90, γ = 120°. The Matthews coefficient and solvent content were calculated as 2.67 Å(3) Da(-1) and 53.97%, respectively. X-ray diffraction data for chIFNGR1 were collected to 2.0 Å resolution at a synchrotron source.
摘要
干扰素-γ（IFN-γ）的活性依赖于信号转导，该信号转导由其与干扰素-γ受体α链（IFNGR1）和β链（IFNGR2）结合触发。天然重组鸡IFNGR1（chIFNGR1；第25至237位氨基酸残基）在大肠杆菌中过表达，经重折叠纯化，并采用气相扩散技术结晶。晶体属于空间群P6(5)22，晶胞参数为a = b = 64.1，c = 216.3 Å，α = β = 90，γ = 120°。马修斯系数和溶剂含量分别计算为2.67 Å(3) Da(-1)和53.97%。chIFNGR1的X射线衍射数据在同步辐射源收集至2.0 Å分辨率。
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2
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Protein Expr Purif. 2009 Dec;68(2):177-82. doi: 10.1016/j.pep.2009.07.006. Epub 2009 Jul 15.
3
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J Interferon Cytokine Res. 2008 Jul;28(7):445-54. doi: 10.1089/jir.2007.0135.
4
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Protein Expr Purif. 2007 Apr;52(2):348-55. doi: 10.1016/j.pep.2006.10.017. Epub 2006 Nov 6.
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Mol Cell. 2003 Dec;12(6):1391-402. doi: 10.1016/s1097-2765(03)00486-6.
8
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10
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